Aptamer-Based Sensor Using Direct Electrochemical for Sensitive Deltamethrin Detection
DOI:
https://doi.org/10.36877/aafrj.a0000549Abstract
A sensitive aptamer sensor based on electrochemical using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were established for detection of deltamethrin. Compared with conventional sensing practicalities, aptamer has many compensations, such as relaxed modification, higher specificity, stronger affinity and well-made stability. The passive deposition of aptamer on gold electrode (SPGE) offered decent biocompatibility and electrical conductivity on the sensor. Following the addition of deltamethrin to the electrode surface, the aptamer is forced to fold, prompting the formation of a 3D structure to specifically interact with deltamethrin. Redox solution was added to encourage the target binding which significantly enhanced the current change of electrochemical signal. Therefore, the auspicious approach provides a signal current and resistance readout using the DPV and EIS technique of aptasensor. In this work, the developed biosensor showed high sensitivity towards deltamethrin via the signal design and autofold target binding. Under the optimized conditions, the anticipated sensor exhibited a good linear regression with R2 value of 0.9932 and 0.9543 via DPV and EIS, respectively for calibration curves of 0.0 to 0.6 ppm. A reproducible and sensitive DPV and EIS on SPGE is developed, reaching a limit of detection of 0.07 ppm (n = 3) and 0.17 ppm (n = 3), respectively compared to the maximum residue limit (MRL) for deltamethrin of 2.0 ppm.
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